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Western Blot analysis of A549 cells using PML Polyclonal Antibody
Western Blot analysis of A549 cells using PML Polyclonal Antibody
Western Blot analysis of A549 cells using PML Polyclonal Antibody

PML Antibody

CSB-PA003823
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPML
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Overview

  • Supplier
    Cusabio
  • Product Name
    PML Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5371
  • Target name
    PML
  • Target description
    PML nuclear body scaffold
  • Target synonyms
    MYL, PP8675, RNF71, TRIM19, protein PML, E3 SUMO-protein ligase PML, PML/RARA fusion, RING finger protein 71, RING-type E3 SUMO transferase PML, probable transcription factor PML, promyelocytic leukemia protein, promyelocytic leukemia, inducer of, tripartite motif protein TRIM19, tripartite motif-containing protein 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29590
  • Protein Name
    Protein PML
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PML using anti-PML antibody (PB10084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human K562 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PML antigen affinity purified polyclonal antibody (Catalog # PB10084) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PML at approximately 90-160 kDa. The expected band size for PML is at 98 kDa.
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