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PMM2 Polyclonal Antibody

BS-12741R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPMM2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PMM2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5373
  • Target name
    PMM2
  • Target description
    phosphomannomutase 2
  • Target synonyms
    CDG1; CDG1a; CDGS; mannose-6-phosphate isomerase; phosphomannomutase 2; phosphomannose isomerase 1; PMI; PMI1; PMM 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15305
  • Protein Name
    Phosphomannomutase 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: PMM2 antibody at 3.8microg/ml Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Hela whole cell lysate Lane 6: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29, 14 kDa Observed band size: 29 kDa
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Figure 1. Western blot analysis of PMM2 using anti-PMM2 antibody (A03146-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat small intestines tissue lysates, Lane 5: mouse small intestines tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMM2 antigen affinity purified polyclonal antibody (Catalog # A03146-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMM2 at approximately 28 kDa. The expected band size for PMM2 is at 28 kDa.
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