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PMPCA Rabbit pAb

A9965
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPMPCA
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Overview

  • Supplier
    ABclonal
  • Product Name
    PMPCA Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23203
  • Target name
    PMPCA
  • Target description
    peptidase, mitochondrial processing subunit alpha
  • Target synonyms
    Alpha-MPP, CLA1, CPD3, INPP5E, MAS2, P-55, SCAR2, mitochondrial-processing peptidase subunit alpha, Cerebellar ataxia-1, cerebellar ataxia 1 (autosomal recessive), inactive zinc metalloprotease alpha, inositol polyphosphate-5-phosphatase, 72 kD, mitochondrial matrix processing protease, alpha subunit, peptidase, mitochondrial processing alpha subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ10713
  • Protein Name
    Mitochondrial-processing peptidase subunit alpha
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PMPCA/INPP5 using anti-PMPCA/INPP5 antibody (A09623-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMPCA/INPP5 antigen affinity purified polyclonal antibody (Catalog # A09623-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMPCA/INPP5 at approximately 50-58 kDa. The expected band size for PMPCA/INPP5 is at 58 kDa.
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