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Western blot All lanes: PMPCB antibody at 2.3microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
Western blot All lanes: PMPCB antibody at 2.3microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
Western blot All lanes: PMPCB antibody at 2.3microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa

PMPCB Antibody

CSB-PA018243ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPMPCB
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Overview

  • Supplier
    Cusabio
  • Product Name
    PMPCB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9512
  • Target name
    PMPCB
  • Target description
    peptidase, mitochondrial processing subunit beta
  • Target synonyms
    Beta-MPP, MAS1, MPP11, MPPB, MPPP52, P-52, mitochondrial-processing peptidase subunit beta, mitochondrial processing peptidase beta subunit, peptidase (mitochondrial processing) beta, peptidase, mitochondrial processing beta subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75439
  • Protein Name
    Mitochondrial-processing peptidase subunit beta
  • Scientific Description
    Cleaves presequences (transit peptides) from mitochondrial protein precursors.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MPPB/PMPCB using anti-MPPB/PMPCB antibody (A11793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPPB/PMPCB antigen affinity purified polyclonal antibody (Catalog # A11793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPPB/PMPCB at approximately 43-54 kDa. The expected band size for MPPB/PMPCB is at 54 kDa.
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