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Hela cell lysates probed with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Hela cell lysates probed with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Hela cell lysates probed with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.

Melan A Recombinant Antibody

BSM-52337R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMLANA
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Overview

  • Supplier
    Bioss
  • Product Name
    PMS2 (9H11) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:50-200), IHC-F(IHC-F=1:50-200), IF(IF=1:50-200), Flow-Cyt(Flow-Cyt=1:50-100), ICC/IF(ICC/IF=1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID2315
  • Target name
    MLANA
  • Target description
    melan-A
  • Target synonyms
    MART-1, MART1, melanoma antigen recognized by T-cells 1, antigen LB39-AA, antigen SK29-AA, epididymis secretory sperm binding protein, protein Melan-A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16655
  • Protein Name
    Melanoma antigen recognized by T-cells 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Melan-A/MLANA using anti-Melan-A/MLANA antibody (A02033-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Melan-A/MLANA antigen affinity purified polyclonal antibody (Catalog # A02033-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Melan-A/MLANA at approximately 13 kDa. The expected band size for Melan-A/MLANA is at 13 kDa.
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