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ProductsBack/PMVK antibody [AT21C10]
ICC/IF analysis of PMVK in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PMVK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of PMVK in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PMVK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of PMVK in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PMVK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

PMVK antibody [AT21C10]

GTX31956
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetPMVK
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Overview

  • Supplier
    GeneTex
  • Product Name
    PMVK antibody [AT21C10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:100
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT21C10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10654
  • Target name
    PMVK
  • Target description
    phosphomevalonate kinase
  • Target synonyms
    HUMPMKI; phosphomevalonate kinase; PMK; PMKA; PMKASE; POROK1; testis tissue sperm-binding protein Li 95mP
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ15126
  • Protein Name
    Phosphomevalonate kinase
  • Scientific Description
    This gene encodes a peroxisomal enzyme that is a member of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family of ATP-dependent enzymes. The encoded protein catalyzes the conversion of mevalonate 5-phosphate to mevalonate 5-diphosphate, which is the fifth step in the mevalonate pathway of isoprenoid biosynthesis. Mutations in this gene are linked to certain types of porokeratosis including disseminated superficial porokeratosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PMVK using anti-PMVK antibody (A10103). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMVK antigen affinity purified polyclonal antibody (Catalog # A10103) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMVK at approximately 22 kDa. The expected band size for PMVK is at 22 kDa.
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