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ProductsBack/PNLIPRP2 Antibody

PNLIPRP2 Antibody

LS-C747964
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPNLIPRP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PNLIPRP2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 51kDa, while the observed MW by Western blot was 54kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) The predicted MW is 51kDa, while the observed MW by Western blot was 54kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5408
  • Target name
    PNLIPRP2
  • Target description
    pancreatic lipase related protein 2 (gene/pseudogene)
  • Target synonyms
    PLRP2, pancreatic lipase-related protein 2, cytotoxic T lymphocyte lipase, galactolipase, triacylglycerol lipase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: PNLIPRP2 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
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Figure 1. Western blot analysis of PNLIPRP2 using anti-PNLIPRP2 antibody (A11017-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNLIPRP2 antigen affinity purified polyclonal antibody (Catalog # A11017-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNLIPRP2 at approximately 52 kDa. The expected band size for PNLIPRP2 is at 52 kDa.
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