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Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PNMT Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PNMT Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PNMT Polyclonal Antibody, Unconjugated (bs-3912R) at 1:200 overnight at 4°C, DAB staining.

PNMT Polyclonal Antibody

BS-3912R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetPNMT
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Overview

  • Supplier
    Bioss
  • Product Name
    PNMT Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5409
  • Target name
    PNMT
  • Target description
    phenylethanolamine N-methyltransferase
  • Target synonyms
    PENT, PNMTase, phenylethanolamine N-methyltransferase, noradrenaline N-methyltransferase, phenylethanolamine N-methylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11086
  • Protein Name
    Phenylethanolamine N-methyltransferase
  • Reactivity
    Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Yue Y, Huo F, Yin C. Noradrenaline-Specific, Efficient Visualization in Brain Tissue Triggered by Unique Cascade Nucleophilic Substitution. Anal Chem. 2019,91(3):2255-2259. doi: 10.1021/acs.analchem.8b04836
    Read this paper
  • Kawahata I, Yamakuni T. Imidacloprid, a neonicotinoid insecticide, facilitates tyrosine hydroxylase transcription and phenylethanolamine N-methyltransferase mRNA expression to enhance catecholamine synthesis and its nicotine-evoked elevation in PC12D cells. Toxicology. 2018,394:84-92. doi: 10.1016/j.tox.2017.12.004
    Read this paper

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