pNPP Phosphatase Assay Kit (Colorimetric)

LifeSpan BioSciences

pNPP Phosphatase Assay Kit (Colorimetric)

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For quantitative determination of phosphatase activity.

Spectrophotometry (visible) For quantitative determination of phosphatase activity.

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p-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer. This homogeneous mix-and-measure assay involves simply adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37C.

2°C to 8°C

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