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The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA275832(PNPT1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA275832(PNPT1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA275832(PNPT1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

PNPT1 Antibody

CSB-PA275832
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPNPT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PNPT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID87178
  • Target name
    PNPT1
  • Target description
    polyribonucleotide nucleotidyltransferase 1
  • Target synonyms
    3'-5' RNA exonuclease OLD35; COXPD13; DFNB70; OLD35; old-35; PNPASE; PNPase 1; PNPase old-35; polynucleotide phosphorylase 1; polynucleotide phosphorylase-like protein; polyribonucleotide nucleotidyltransferase 1, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TCS8
  • Protein Name
    Polyribonucleotide nucleotidyltransferase 1, mitochondrial
  • Scientific Description
    The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3-to-5 exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PNPT1 using anti-PNPT1 antibody (A05460-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNPT1 antigen affinity purified polyclonal antibody (Catalog # A05460-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNPT1 at approximately 86KD. The expected band size for PNPT1 is at 86KD.
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