Western blot using GeneTex Immuno-chemicals affinity purified anti-Pogz antibody shows detection of Pogz protein (arrowhead) in adult mouse thymus and spleen tissue lysate (Panel A). The lower molecular weight bands may be cross reactive proteins. Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity (Panel B). Primary antibody was used at 1:20,000.
Western blot using GeneTex Immuno-chemicals affinity purified anti-Pogz antibody shows detection of Pogz protein (arrowhead) in adult mouse thymus and spleen tissue lysate (Panel A). The lower molecular weight bands may be cross reactive proteins. Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity (Panel B). Primary antibody was used at 1:20,000.
Pogz antibody
GTX48710
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetPogz
Overview
- SupplierGeneTex
- Product NamePogz antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000-1:20000. ELISA: 1:100000-1:400000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.6 mg/ml
- ConjugateUnconjugated
- Gene ID229584
- Target namePogz
- Target descriptionpogo transposable element with ZNF domain
- Target synonyms9530006B08Rik, pogo transposable element with ZNF domain
- HostRabbit
- IsotypeIgG
- Protein IDQ8BZH4
- Protein NamePogo transposable element with ZNF domain
- Scientific DescriptionPogz is a zinc-finger protein containing at least 8 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. Members of this family of integrases share structural homologies and show similar catalytic activity to the RAG1 and RAG2 proteins that initiate V(D)J recombination in lymphoid progenitors. Mouse Pogz has a human homolog with a base pair similarity of 90% and amino acid similarity of 93% for the full length protein. In humans at least 3 variants have been predicted and they are all similar at the N-terminus. A number of expressed sequence tags (ESTs) cloned from murine undifferentiated ES cells and Lin-/c-Kit+/Sca-1+ hematopoietic stem cell cDNA libraries correspond to the Pogz gene, further underlining an important function for this gene in stem cells. In mice, deletion of the gene in all tissues early in embryogenesis has been shown to be lethal.
- ReactivityMouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161