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ProductsBack/POLA2 / DNA Polymerase Alpha 2 Antibody

POLA2 / DNA Polymerase Alpha 2 Antibody

LS-C332545
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPOLA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    POLA2 / DNA Polymerase Alpha 2 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:3000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23649
  • Target name
    POLA2
  • Target description
    DNA polymerase alpha 2, accessory subunit
  • Target synonyms
    DNA polymerase alpha subunit B, DNA polymerase alpha 70 kDa subunit, polymerase (DNA directed), alpha 2 (70kD subunit), polymerase (DNA directed), alpha 2, accessory subunit, polymerase (DNA) alpha 2, accessory subunit, polymerase (DNA-directed), alpha (70kD)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLA2 using anti-POLA2 antibody (A08427-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human COLO320 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLA2 antigen affinity purified polyclonal antibody (Catalog # A08427-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLA2 at approximately 70 kDa. The expected band size for POLA2 is at 66 kDa.
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