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ProductsBack/POLDIP2 / PDIP38 Antibody (Biotin)

POLDIP2 / PDIP38 Antibody (Biotin)

LS-C398147
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetPOLDIP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    POLDIP2 / PDIP38 Antibody (Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID26073
  • Target name
    POLDIP2
  • Target description
    DNA polymerase delta interacting protein 2
  • Target synonyms
    PDIP38, p38, polymerase delta-interacting protein 2, 38 kDa DNA polymerase delta interaction protein, polymerase (DNA) delta interacting protein 2, polymerase (DNA-directed), delta interacting protein 2, polymerase delta interacting protein 38
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLDIP2 using anti-POLDIP2 antibody (A09581-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLDIP2 antigen affinity purified polyclonal antibody (Catalog # A09581-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLDIP2 at approximately 36 kDa. The expected band size for POLDIP2 is at 42 kDa.
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