WB analysis of various samples using GTX00871 POLH antibody [5H10]. Lane 1 : Recombinant full-size Pol eta (POLH) with 6XHis tag at C-teminus Lnae 2 : HeLa whole cell lysate (~40 microg) Lane 3 : XP2SA (XP-V) whole cell lysate (~40 microg)
![ICC/IF analysis of HeLa cells using GTX00871 POLH antibody [5H10]. Fixation : 4% PFA, overnight Permeabilization : 0.25% Triton X-100 in PBS, 10 min Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX00871/GTX00871_20200406_AP_002_84_w_23053121_631.webp "ICC/IF analysis of HeLa cells using GTX00871 POLH antibody [5H10]. Fixation : 4% PFA, overnight Permeabilization : 0.25% Triton X-100 in PBS, 10 min Dilution : 1:1000")
WB analysis of various samples using GTX00871 POLH antibody [5H10]. Lane 1 : Recombinant full-size Pol eta (POLH) with 6XHis tag at C-teminus Lnae 2 : HeLa whole cell lysate (~40 microg) Lane 3 : XP2SA (XP-V) whole cell lysate (~40 microg)
POLH antibody [5H10]
GTX00871
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPOLH
Overview
- SupplierGeneTex
- Product NamePOLH antibody [5H10]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ICC/IF: 1:200-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5h10
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5429
- Target namePOLH
- Target descriptionDNA polymerase eta
- Target synonymsDNA polymerase eta; polymerase (DNA directed), eta; polymerase (DNA) eta; RAD30; RAD30 homolog A; RAD30A; xeroderma pigmentosum variant type protein; XPV; XP-V
- HostMouse
- IsotypeIgG1
- Protein IDQ9Y253
- Protein NameDNA polymerase eta
- Scientific DescriptionThis gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203