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Western Blot Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate All lanes: POLH antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 79, 47 kDa Observed band size: 79 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate All lanes: POLH antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 79, 47 kDa Observed band size: 79 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate All lanes: POLH antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 79, 47 kDa Observed band size: 79 kDa

POLH Antibody

CSB-PA018321LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPOLH
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Overview

  • Supplier
    Cusabio
  • Product Name
    POLH Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5429
  • Target name
    POLH
  • Target description
    DNA polymerase eta
  • Target synonyms
    RAD30, RAD30A, XP-V, XPV, DNA polymerase eta, DNA polymerase eta transcript, RAD30 homolog A, polymerase (DNA directed), eta, polymerase (DNA) eta, xeroderma pigmentosum variant type protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y253
  • Protein Name
    DNA polymerase eta
  • Scientific Description
    DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POLH using anti-POLH antibody (PB9813). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLH antigen affinity purified polyclonal antibody (Catalog # PB9813) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLH at approximately 78 kDa. The expected band size for POLH is at 78 kDa.
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