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WB analysis of A375 (A), HEK293T (B), NIH3T3 (C), H9C2 (D) whole cell lysates using GTX55943 POLR1A antibody.
WB analysis of A375 (A), HEK293T (B), NIH3T3 (C), H9C2 (D) whole cell lysates using GTX55943 POLR1A antibody.
WB analysis of A375 (A), HEK293T (B), NIH3T3 (C), H9C2 (D) whole cell lysates using GTX55943 POLR1A antibody.

POLR1A antibody

GTX55943
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPOLR1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    POLR1A antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25885
  • Target name
    POLR1A
  • Target description
    RNA polymerase I subunit A
  • Target synonyms
    A190; AFDCIN; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; DNA-directed RNA polymerase I subunit A1; DNA-directed RNA polymerase I subunit RPA1; polymerase (RNA) I polypeptide A, 194kDa; polymerase (RNA) I subunit A; RNA polymerase I 194 kDa subunit; RPA1; RPA190; RPA194; RPO14; RPO1-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95602
  • Protein Name
    DNA-directed RNA polymerase I subunit RPA1
  • Scientific Description
    The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR1A using anti-POLR1A antibody (A10549-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1A antigen affinity purified polyclonal antibody (A10549-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR1A at approximately 195 kDa. The expected band size for POLR1A is at 195 kDa.
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