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POLR1B Antibody

LS-C747242
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPOLR1B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    POLR1B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 104kDa/107kDa/121kDa/128kDa/132kDa, while the observed MW by Western blot was 120kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 104kDa/107kDa/121kDa/128kDa/132kDa, while the observed MW by Western blot was 120kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84172
  • Target name
    POLR1B
  • Target description
    RNA polymerase I subunit B
  • Target synonyms
    A135, RPA135, RPA2, Rpo1-2, TCS4, DNA-directed RNA polymerase I subunit RPA2, DNA-directed RNA polymerase I 135 kDa polypeptide, DNA-directed RNA polymerase I 135kDa polypeptide, RNA polymerase I subunit 2, polymerase (RNA) I polypeptide B, 128kDa, polymerase (RNA) I subunit B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR1B using anti-POLR1B antibody (A11908-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1B antigen affinity purified polyclonal antibody (Catalog # A11908-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR1B at approximately 128 kDa. The expected band size for POLR1B is at 128 kDa.
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