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POLR3D Antibody

CSB-PA050109
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPOLR3D
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Overview

  • Supplier
    Cusabio
  • Product Name
    POLR3D Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID661
  • Target name
    POLR3D
  • Target description
    RNA polymerase III subunit D
  • Target synonyms
    BN51 (BHK21) temperature sensitivity complementing; BN51T; C53; DNA-directed RNA polymerase III 47 kDa polypeptide; DNA-directed RNA polymerase III subunit D; DNA-directed RNA polymerase III subunit RPC4; polymerase (RNA) III (DNA directed) polypeptide D, 44kDa; polymerase (RNA) III subunit D; RNA polymerase III 47 kDa subunit; RNA polymerase III subunit C4; RPC4; RPC53; RPC53 homolog; temperature sensitive complementation, cell cycle specific, tsBN51; TSBN51
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05423
  • Protein Name
    DNA-directed RNA polymerase III subunit RPC4
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR3D using anti-POLR3D antibody (A11611-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR3D antigen affinity purified polyclonal antibody (Catalog # A11611-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR3D at approximately 44 kDa. The expected band size for POLR3D is at 44 kDa.
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TargetPOLR3D
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