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POLR3D Antibody

CSB-PA050109
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetPOLR3D
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Overview

  • Supplier
    Cusabio
  • Product Name
    POLR3D Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID661
  • Target name
    POLR3D
  • Target description
    RNA polymerase III subunit D
  • Target synonyms
    BN51T, C53, RPC4, RPC53, TSBN51, DNA-directed RNA polymerase III subunit RPC4, BN51 (BHK21) temperature sensitivity complementing, DNA-directed RNA polymerase III 47 kDa polypeptide, DNA-directed RNA polymerase III subunit D, RNA polymerase III 47 kDa subunit, RNA polymerase III subunit C4, RPC53 homolog, polymerase (RNA) III (DNA directed) polypeptide D, 44kDa, polymerase (RNA) III subunit D, temperature sensitive complementation, cell cycle specific, tsBN51
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05423
  • Protein Name
    DNA-directed RNA polymerase III subunit RPC4
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POLR3D using anti-POLR3D antibody (A11611-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR3D antigen affinity purified polyclonal antibody (Catalog # A11611-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR3D at approximately 44 kDa. The expected band size for POLR3D is at 44 kDa.
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