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Western blot All lanes: PON1 antibody at 2microg/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
Western blot All lanes: PON1 antibody at 2microg/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
Western blot All lanes: PON1 antibody at 2microg/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa

PON1 Antibody

CSB-PA018369EA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPON1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PON1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5444
  • Target name
    PON1
  • Target description
    paraoxonase 1
  • Target synonyms
    A-esterase 1; aromatic esterase 1; arylesterase 1; arylesterase B-type; ESA; esterase A; K-45; MVCD5; paraoxonase B-type; PON; PON 1; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1; serum paraoxonase/arylesterase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27169
  • Protein Name
    Serum paraoxonase/arylesterase 1
  • Scientific Description
    Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PON1 using anti-PON1 antibody (A00516-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON1 antigen affinity purified polyclonal antibody (Catalog # A00516-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PON1 at approximately 40KD. The expected band size for PON1 is at 40KD.
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