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Western blot analysis of PON1 (arrow) using PON1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PON1 gene (Lane 2).
Western blot analysis of PON1 (arrow) using PON1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PON1 gene (Lane 2).
Western blot analysis of PON1 (arrow) using PON1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PON1 gene (Lane 2).

PON1 (Center) Rabbit Polyclonal Antibody

AP53390PU-N
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPON1
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PON1 (Center) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5444
  • Target name
    PON1
  • Target description
    paraoxonase 1
  • Target synonyms
    ESA, MVCD5, PON, serum paraoxonase/arylesterase 1, A-esterase 1, K-45, PON 1, aromatic esterase 1, arylesterase 1, arylesterase B-type, esterase A, paraoxonase B-type, serum aryldiakylphosphatase, serum aryldialkylphosphatase 1
  • Host
    Rabbit
  • Protein IDP27169
  • Protein Name
    Serum paraoxonase/arylesterase 1
  • Scientific Description
    PON1 (Center) rabbit polyclonal antibody, Aff - Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PON1 using anti-PON1 antibody (A00516-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON1 antigen affinity purified polyclonal antibody (Catalog # A00516-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PON1 at approximately 40KD. The expected band size for PON1 is at 40KD.
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