Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with POU6F1/BRN5 Polyclonal Antibody, Unconjugated (bs-21049R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with POU6F1/BRN5 Polyclonal Antibody, Unconjugated (bs-21049R) at 1:200 overnight at 4°C, DAB staining.
POU6F1/BRN5 Polyclonal Antibody
BS-21049R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetPOU6F1
Overview
- SupplierBioss
- Product NamePOU6F1/BRN5 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID5463
- Target namePOU6F1
- Target descriptionPOU class 6 homeobox 1
- Target synonymsBRN5, MPOU, TCFB1, POU domain, class 6, transcription factor 1, brain-5, brain-specific homeobox/POU domain protein 5, mPOU homeobox protein
- HostRabbit
- IsotypeIgG
- Protein IDQ14863
- Protein NamePOU domain, class 6, transcription factor 1
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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