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PPAN Antibody

LS-C349098
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPPAN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PPAN Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 51kDa/53kDa/58kDa, while the observed MW by Western blot was 55kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 51kDa/53kDa/58kDa, while the observed MW by Western blot was 55kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID56342
  • Target name
    PPAN
  • Target description
    peter pan homolog
  • Target synonyms
    BXDC3, SSF, SSF-1, SSF1, SSF2, suppressor of SWI4 1 homolog, brix domain-containing protein 3, homolog of S. cerevisiae SSF1, second-step splicing factor 1, suppressor of sterile four 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPAN using anti-PPAN antibody (A10836-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPAN antigen affinity purified polyclonal antibody (Catalog # A10836-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPAN at approximately 65 kDa. The expected band size for PPAN is at 53 kDa.
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