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Formalin-fixed and paraffin-embedded human skin psoriasis labeled with Rabbit Anti- PPARr gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human skin psoriasis labeled with Rabbit Anti- PPARr gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human skin psoriasis labeled with Rabbit Anti- PPARr gamma Polyclonal Antibody, Unconjugated (bs-0530R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

PPAR gamma Polyclonal Antibody

BS-0530R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPPARG
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PPAR gamma Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5468
  • Target name
    PPARG
  • Target description
    peroxisome proliferator activated receptor gamma
  • Target synonyms
    CIMT1; GLM1; NR1C3; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated nuclear receptor gamma variant 1; peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor-gamma 5; peroxisome proliferator-activated receptor-gamma splicing; PPARG1; PPARG2; PPARG5; PPARgamma; PPAR-gamma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP37231
  • Protein Name
    Peroxisome proliferator-activated receptor gamma
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Dong S et al., 2019 Jun 15, Life Sci
    Read more
  • Resistin up-regulates LPL expression through the PPARgamma-dependent PI3K/AKT signaling pathway impacting lipid accumulation in RAW264.7 macrophages. Li B et al., 2019 Jul, Cytokine
    Read more
  • Insulin-like growth factor 2 promotes the adipogenesis of hemangioma-derived stem cells. Zhang K et al., 2019 Mar, Exp Ther Med
    Read more
  • Insulin-like growth factor I promotes adipogenesis in hemangioma stem cells from infantile hemangiomas. Wang F et al., 2019 Apr, Mol Med Rep
    Read more
  • Upregulation of transient receptor potential melastatin 6 channel expression by rosiglitazone and all-trans-retinoic acid in erlotinib-treated renal tubular epithelial cells. Manabe A et al., 2019 Jun, J Cell Physiol
    Read more
  • Melatonin promotes triacylglycerol accumulation via MT2 receptor during differentiation in bovine intramuscular preadipocytes. Yang W et al., 2017 Nov 8, Sci Rep
    Read more
  • Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPbeta and PPARgamma mediated lipoapoptosis. Li H et al., 2015 Nov 4, J Exp Clin Cancer Res
    Read more
  • 1,25-Dihydroxyvitamin D3 Promotes High Glucose-Induced M1 Macrophage Switching to M2 via the VDR-PPARgamma Signaling Pathway. Zhang X et al., 2015, Biomed Res Int
    Read more
  • Correlation between TSP-1, TGF-beta and PPAR-gamma expression levels and glioma microvascular density. Zhang J et al., 2014 Jan, Oncol Lett
    Read more

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Figure 1. Western blot analysis of PPAR gamma using anti-PPAR gamma antibody (A00449-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPAR gamma antigen affinity purified polyclonal antibody (Catalog # A00449-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPAR gamma at approximately 58 kDa. The expected band size for PPAR gamma is at 58 kDa.
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