PPARalpha (Peroxisome proliferator-activated receptor alpha)-GAL4 Luciferase Reporter HEK293 Cell Line

BPS Bioscience

PPARalpha (Peroxisome proliferator-activated receptor alpha)-GAL4 Luciferase Reporter HEK293 Cell Line

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Research Use Only

The PPARalpha (Peroxisome proliferator-activated receptor alpha)-GAL4 Luciferase Reporter HEK293 Cell Lineexpresses firefly luciferase under the control of the GAL4 upstream activation sequence (UAS). It has constitutive expression of human peroxisome proliferator-activated receptor alpha ligand binding domain (PPARalpha LBD, amino acids 166-468) fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD). This system allows specific measurement of the effect of PPARalpha agonists with low cross-reactivity from other nuclear receptors or from other PPAR isoforms. This cell line has been validated by stimulation with GW-7647, and for its lack of stimulation to Rosiglitazone and GW501516. Figure 1: Illustration of the mechanism leading to luciferase induction upon agonist treatment in PPARalpha (Peroxisome proliferator-activated receptor alpha)-GAL4 Luciferase Reporter HEK293 Cell Line. Interested in screening and profiling inhibitors, blocking antibodies, or activators of PPARa without the need to purchase and license the cell line?/ Check out our Cell Signaling Pathway Screening.

Liquid Nitrogen

12352207