PPARalpha (Peroxisome proliferator-activated receptor alpha)-GAL4 Luciferase Reporter HEK293 Cell Line

BPS Bioscience

PPARalpha (Peroxisome proliferator-activated receptor alpha)-GAL4 Luciferase Reporter HEK293 Cell Line

23

Research Use Only

PPARA

peroxisome proliferator activated receptor alpha

NR1C1, PPAR, PPAR-alpha, PPARalpha, hPPAR, peroxisome proliferator-activated receptor alpha, nuclear receptor subfamily 1 group C member 1, peroxisome proliferative activated receptor, alpha

Peroxisome proliferator-activated receptor alpha

The PPARalpha (Peroxisome proliferator-activated receptor alpha)-GAL4 Luciferase Reporter HEK293 Cell Lineexpresses firefly luciferase under the control of the GAL4 upstream activation sequence (UAS). It has constitutive expression of human peroxisome proliferator-activated receptor alpha ligand binding domain (PPARalpha LBD, amino acids 166-468) fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD). This system allows specific measurement of the effect of PPARalpha agonists with low cross-reactivity from other nuclear receptors or from other PPAR isoforms. This cell line has been validated by stimulation with GW-7647, and for its lack of stimulation to Rosiglitazone and GW501516. Figure 1: Illustration of the mechanism leading to luciferase induction upon agonist treatment in PPARalpha (Peroxisome proliferator-activated receptor alpha)-GAL4 Luciferase Reporter HEK293 Cell Line. Interested in screening and profiling inhibitors, blocking antibodies, or activators of PPARa without the need to purchase and license the cell line?/ Check out our Cell Signaling Pathway Screening.

Liquid Nitrogen

41116106

Human