PPARgamma (Peroxisome proliferator-activated receptor gamma)-GAL4 Luciferase Reporter HEK293 Cell Line

BPS Bioscience

PPARgamma (Peroxisome proliferator-activated receptor gamma)-GAL4 Luciferase Reporter HEK293 Cell Line

23

Research Use Only

The PPARgamma (Peroxisome proliferator-activated receptor gamma)-GAL4 Luciferase Reporter HEK293 Cell Line expresses firefly luciferase under the control of the GAL4 upstream activation sequence (UAS). It has constitutive expression of human peroxisome proliferator-activated receptor gamma ligand binding domain (PPARgamma LBD, amino acids 173-475) fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD). This system allows specific measurement of the effect of PPARgamma agonists with low cross-reactivity from other nuclear receptors or from other PPAR isoforms. This cell line has been validated by stimulation with Rosiglitazone, and for its lack of stimulation to GW7647 and GW501516. Figure 1: Illustration of the mechanism leading to luciferase induction upon agonist treatment in PPARgamma (Peroxisome proliferator-activated receptor gamma)-GAL4 Luciferase Reporter HEK293 Cell Line. Interested in screening and profiling inhibitors, blocking antibodies, or activators of PPAR without the need to purchase and license the cell line?/ Check out our Cell Signaling Pathway Screening.

Liquid Nitrogen

12352207