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ProductsBack/PPCS antibody [N2C3]

PPCS antibody [N2C3]

GTX119988
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPPCS
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Overview

  • Supplier
    GeneTex
  • Product Name
    PPCS antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.69 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID79717
  • Target name
    PPCS
  • Target description
    phosphopantothenoylcysteine synthetase
  • Target synonyms
    CMD2C; phosphopantothenate--cysteine ligase; PPC synthetase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HAB8
  • Protein Name
    Phosphopantothenate--cysteine ligase
  • Scientific Description
    Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPCS using anti-PPCS antibody (A05567-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPCS antigen affinity purified polyclonal antibody (Catalog # A05567-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPCS at approximately 35 kDa. The expected band size for PPCS is at 34 kDa.
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