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PPIL3 Antibody

LS-C814989
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPPIL3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PPIL3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID53938
  • Target name
    PPIL3
  • Target description
    peptidylprolyl isomerase like 3
  • Target synonyms
    CYPJ, peptidyl-prolyl cis-trans isomerase-like 3, PPIase, PPIase-like protein 3, cyclophilin J, cyclophilin-like protein 3, cyclophilin-like protein PPIL3, peptidylprolyl cis-trans isomerase-like protein 3, peptidylprolyl isomerase (cyclophilin)-like 3, rotamase PPIL3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: PPIL3 antibody at 10microg/ml Lane 1: U87 whole cell lysate Lane 2: HL60 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19 kDa Observed band size: 19 kDa
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Figure 1. Western blot analysis of PPIL3 using anti-PPIL3 antibody (A09246-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIL3 antigen affinity purified polyclonal antibody (Catalog # A09246-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPIL3 at approximately 18 kDa. The expected band size for PPIL3 is at 18 kDa.
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