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Western blot All lanes: PPM1A antibody at 6.88microg/ml Lane 1: HepG2 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: Mouse liver tissue Lane 4: Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 36, 52 kDa Observed band size: 43 kDa
Western blot All lanes: PPM1A antibody at 6.88microg/ml Lane 1: HepG2 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: Mouse liver tissue Lane 4: Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 36, 52 kDa Observed band size: 43 kDa
Western blot All lanes: PPM1A antibody at 6.88microg/ml Lane 1: HepG2 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: Mouse liver tissue Lane 4: Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 36, 52 kDa Observed band size: 43 kDa

PPM1A Antibody

CSB-PA018489DSR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPPM1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPM1A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5494
  • Target name
    PPM1A
  • Target description
    protein phosphatase, Mg2+/Mn2+ dependent 1A
  • Target synonyms
    PP2CA; PP2Calpha; PP2C-ALPHA; protein phosphatase 1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35813
  • Protein Name
    Protein phosphatase 1A
  • Scientific Description
    Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPM1A using anti-PPM1A antibody (A02928-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1A antigen affinity purified polyclonal antibody (Catalog # A02928-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1A at approximately 40 kDa. The expected band size for PPM1A is at 42 kDa.
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