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ProductsBack/PPM1G (Center) Rabbit Polyclonal Antibody
Western Blot analysis: PPM1G antibody staining of 293 cell line lysate (Left) and Mouse heart tissue lysate (Right) (35 ug/lane).
Western Blot analysis: PPM1G antibody staining of 293 cell line lysate (Left) and Mouse heart tissue lysate (Right) (35 ug/lane).
Western Blot analysis: PPM1G antibody staining of 293 cell line lysate (Left) and Mouse heart tissue lysate (Right) (35 ug/lane).

PPM1G (Center) Rabbit Polyclonal Antibody

AP15246PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetPPM1G
Price on request
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PPM1G (Center) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5496
  • Target name
    PPM1G
  • Target description
    protein phosphatase, Mg2+/Mn2+ dependent 1G
  • Target synonyms
    PP2CG, PP2CGAMMA, PPP2CG, protein phosphatase 1G, PP2C-gamma, protein phosphatase 1C, protein phosphatase magnesium-dependent 1 gamma
  • Host
    Rabbit
  • Protein IDO15355
  • Protein Name
    Protein phosphatase 1G
  • Scientific Description
    PPM1G (Center) rabbit polyclonal antibody, Purified
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Cell lysates of 293T(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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Figure 1. Western blot analysis of PPM1G using anti-PPM1G antibody (A06725-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1G antigen affinity purified polyclonal antibody (Catalog # A06725-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1G at approximately 75 kDa. The expected band size for PPM1G is at 59 kDa.
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