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ProductsBack/PPM1G Mouse Monoclonal Antibody [Clone ID: OTI1C12]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PPM1G (Cat# RC213685, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1G(Cat# TA502232). Positive lysates [LY406071] (100ug) and [LC406071] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PPM1G (Cat# RC213685, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1G(Cat# TA502232). Positive lysates [LY406071] (100ug) and [LC406071] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PPM1G (Cat# RC213685, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1G(Cat# TA502232). Positive lysates [LY406071] (100ug) and [LC406071] (20ug) can be purchased separately from OriGene.

PPM1G Mouse Monoclonal Antibody [Clone ID: OTI1C12]

CF502232
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetPPM1G
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PPM1G Mouse Monoclonal Antibody [Clone ID: OTI1C12]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1C12
  • Gene ID5496
  • Target name
    PPM1G
  • Target description
    protein phosphatase, Mg2+/Mn2+ dependent 1G
  • Target synonyms
    PP2CG, PP2CGAMMA, PPP2CG, protein phosphatase 1G, PP2C-gamma, protein phosphatase 1C, protein phosphatase magnesium-dependent 1 gamma
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDO15355
  • Protein Name
    Protein phosphatase 1G
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PPM1G mouse monoclonal antibody, clone OTI1C12 (formerly 1C12)
  • Reactivity
    Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PPM1G using anti-PPM1G antibody (A06725-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1G antigen affinity purified polyclonal antibody (Catalog # A06725-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1G at approximately 75 kDa. The expected band size for PPM1G is at 59 kDa.
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