More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PPM1J antibody, N-term
WB analysis of tranfected 293T cells using GTX45386 PPM1J antibody at 0.2-1microg/ml.
WB analysis of tranfected 293T cells using GTX45386 PPM1J antibody at 0.2-1microg/ml.
WB analysis of tranfected 293T cells using GTX45386 PPM1J antibody at 0.2-1microg/ml.

PPM1J antibody, N-term

GTX45386
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPPM1J
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    PPM1J antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID333926
  • Target name
    PPM1J
  • Target description
    protein phosphatase, Mg2+/Mn2+ dependent 1J
  • Target synonyms
    PP2CZ; PP2Czeta; PP2C-zeta; PPP2CZ; protein phosphatase 1J; protein phosphatase 1J (PP2C domain containing); protein phosphatase 2C zeta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5JR12
  • Protein Name
    Protein phosphatase 1J
  • Scientific Description
    This gene encodes the serine/threonine protein phosphatase. The mouse homolog of this gene apparently belongs to the protein phosphatase 2C family of genes. The exact function of this gene is not yet known. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PPM1J antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 34 kDa Observed band size: 55 kDa
Product group Antibodies
Cusabio
PPM1J AntibodyCSB-PA686382LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetPPM1J
  • SizePrice
Product group Antibodies
Biomatik
PPM1J Polyclonal AntibodyCAC13365
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetPPM1J
  • SizePrice
Product group Antibodies
GeneTex
PPM1J antibodyGTX103370
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPPM1J
  • SizePrice
WB analysis of human B lymphoblast cells using GTX45385 PPM1J antibody at 0.2-1microg/ml.
Product group Antibodies
GeneTex
PPM1J antibody, C-termGTX45385
ApplicationsWestern Blot
TargetPPM1J
  • SizePrice
Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
Product group Antibodies
Atlas Antibodies
Anti-PPM1J AntibodyHPA046045
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
TargetPPM1J
  • SizePrice
Anti-PPM1J Antibody
Product group Antibodies
Antibodies.com
Anti-PPM1J AntibodyA90669
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PPM1J Antibody144-12844
ApplicationsWestern Blot
TargetPPM1J
  • SizePrice
Figure 1. Western blot analysis of PPM1J using anti-PPM1J antibody (A15780-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1J antigen affinity purified polyclonal antibody (Catalog # A15780-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1J at approximately 55 kDa. The expected band size for PPM1J is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-PPM1J Antibody Picoband(r)A15780-2-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetPPM1J
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PPM1J AntibodyLS-C747913
ApplicationsWestern Blot
TargetPPM1J
  • SizePrice