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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA03834A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA03834A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA03834A0Rb at dilution of 1:100

PPP1R11 Antibody

CSB-PA03834A0RB
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPP1R11
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPP1R11 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6992
  • Target name
    PPP1R11
  • Target description
    protein phosphatase 1 regulatory inhibitor subunit 11
  • Target synonyms
    CFAP255, HCG-V, HCGV, IPP3, TCTE5, TCTEX5, E3 ubiquitin-protein ligase PPP1R11, HCG V, hemochromatosis candidate gene V protein, inhibitor-3, protein phosphatase 1 regulatory subunit 11, protein phosphatase inhibitor 3, t-complex-associated-testis-expressed 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60927
  • Protein Name
    E3 ubiquitin-protein ligase PPP1R11
  • Scientific Description
    Atypical E3 ubiquitin-protein ligase which ubiquitinates TLR2 at Lys-754 leading to its degradation by the proteasome. Plays a role in regulating inflammatory cytokine release and gram-positive bacterial clearance by functioning, in part, through the ubiquitination and degradation of TLR2 (PubMed:27805901). Inhibitor of protein phosphatase 1 (PubMed:9843442).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPP1R11 using anti-PPP1R11 antibody (A10474-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R11 antigen affinity purified polyclonal antibody (Catalog # A10474-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R11 at approximately 25 kDa. The expected band size for PPP1R11 is at 14,18,25-30 kDa.
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