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Western Blot analysis of COLO205 cells using KEPI Polyclonal Antibody
Western Blot analysis of COLO205 cells using KEPI Polyclonal Antibody
Western Blot analysis of COLO205 cells using KEPI Polyclonal Antibody

PPP1R14C Antibody

CSB-PA003096
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPP1R14C
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPP1R14C Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81706
  • Target name
    PPP1R14C
  • Target description
    protein phosphatase 1 regulatory inhibitor subunit 14C
  • Target synonyms
    CPI17-like; KEPI; kinase C-enhanced PP1 inhibitor; kinase-enhanced PP1 inhibitor; NY-BR-81; PKC-potentiated PP1 inhibitory protein; protein phosphatase 1 regulatory subunit 14C; serologically defined breast cancer antigen NY-BR-81
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TAE6
  • Protein Name
    Protein phosphatase 1 regulatory subunit 14C
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KEPI/PPP1R14C using anti-KEPI/PPP1R14C antibody (A10973-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat H9C2(2-1) whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KEPI/PPP1R14C antigen affinity purified polyclonal antibody (Catalog # A10973-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KEPI/PPP1R14C at approximately 20 kDa. The expected band size for KEPI/PPP1R14C is at 20 kDa.
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