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PPP1R14C Antibody (OAAB19467)

OAAB19467
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetPPP1R14C
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    PPP1R14C Antibody (OAAB19467)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Format
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Gene ID81706
  • Target name
    PPP1R14C
  • Target description
    protein phosphatase 1 regulatory inhibitor subunit 14C
  • Target synonyms
    CPI17-like; KEPI; kinase C-enhanced PP1 inhibitor; kinase-enhanced PP1 inhibitor; NY-BR-81; PKC-potentiated PP1 inhibitory protein; protein phosphatase 1 regulatory subunit 14C; serologically defined breast cancer antigen NY-BR-81
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KEPI/PPP1R14C using anti-KEPI/PPP1R14C antibody (A10973-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat H9C2(2-1) whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KEPI/PPP1R14C antigen affinity purified polyclonal antibody (Catalog # A10973-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KEPI/PPP1R14C at approximately 20 kDa. The expected band size for KEPI/PPP1R14C is at 20 kDa.
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