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Western Blot analysis of RAT-MUSLE cells using DARPP-32 Polyclonal Antibody
Western Blot analysis of RAT-MUSLE cells using DARPP-32 Polyclonal Antibody
Western Blot analysis of RAT-MUSLE cells using DARPP-32 Polyclonal Antibody

PPP1R1B Antibody

CSB-PA002073
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPPP1R1B
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPP1R1B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84152
  • Target name
    PPP1R1B
  • Target description
    protein phosphatase 1 regulatory inhibitor subunit 1B
  • Target synonyms
    DARPP32; DARPP-32; dopamine and cAMP-regulated neuronal phosphoprotein 32; protein phosphatase 1 regulatory subunit 1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UD71
  • Protein Name
    Protein phosphatase 1 regulatory subunit 1B
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DARPP32 using anti-DARPP32 antibody (PB9879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DARPP32 antigen affinity purified polyclonal antibody (Catalog # PB9879) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DARPP32 at approximately 32 kDa. The expected band size for DARPP32 is at 23 kDa.
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