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PPP3CB Monoclonal Antibody

BSM-60262M
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPPP3CB
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Overview

  • Supplier
    Bioss
  • Product Name
    PPP3CB Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F4G2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5532
  • Target name
    PPP3CB
  • Target description
    protein phosphatase 3 catalytic subunit beta
  • Target synonyms
    CALNA2, CALNB, CNA2, PP2Bbeta, serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, CAM-PRP catalytic subunit, CNA beta, calcineurin A beta, calcineurin A2, protein phosphatase 3, catalytic subunit, beta isozyme, protein phosphatase from PCR fragment H32
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16298
  • Protein Name
    Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP3CB using anti-PPP3CB antibody (A07303-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3CB antigen affinity purified polyclonal antibody (Catalog # A07303-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3CB at approximately 59 kDa. The expected band size for PPP3CB is at 59 kDa.
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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
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