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ProductsBack/PPWD1 Mouse Monoclonal Antibody [Clone ID: OTI1G5]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPWD1 (RC204570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPWD1. Positive lysates LY414604 (100ug) and LC414604 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPWD1 (RC204570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPWD1. Positive lysates LY414604 (100ug) and LC414604 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPWD1 (RC204570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPWD1. Positive lysates LY414604 (100ug) and LC414604 (20ug) can be purchased separately from OriGene.

PPWD1 Mouse Monoclonal Antibody [Clone ID: OTI1G5]

CF502069
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetPPWD1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PPWD1 Mouse Monoclonal Antibody [Clone ID: OTI1G5]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1G5
  • Gene ID23398
  • Target name
    PPWD1
  • Target description
    peptidylprolyl isomerase domain and WD repeat containing 1
  • Target synonyms
    peptidylprolyl isomerase domain and WD repeat-containing protein 1, epididymis secretory sperm binding protein, spliceosome-associated cyclophilin
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ96BP3
  • Protein Name
    Peptidylprolyl isomerase domain and WD repeat-containing protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PPWD1 mouse monoclonal antibody, clone OTI1G5 (formerly 1G5)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PPWD1 using anti-PPWD1 antibody (A11798-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPWD1 antigen affinity purified polyclonal antibody (Catalog # A11798-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPWD1 at approximately 70 kDa. The expected band size for PPWD1 is at 70 kDa.
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