Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Ser183) antibody [HL3189] (GTX640828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Ser183) antibody [HL3189] (GTX640828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PRAS40 (phospho Ser183) antibody [HL3189]
GTX640828
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetAKT1S1
Overview
- SupplierGeneTex
- Product NamePRAS40 (phospho Ser183) antibody [HL3189]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3189
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID84335
- Target nameAKT1S1
- Target descriptionAKT1 substrate 1
- Target synonymsLobe, PRAS40, proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate, AKT1 substrate 1 (proline rich)
- HostRabbit
- IsotypeIgG
- Protein IDQ96B36
- Protein NameProline-rich AKT1 substrate 1
- Scientific DescriptionAKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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