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ProductsBack/PRD Rabbit mAb

PRD Rabbit mAb

ORB1950293
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
TargetPEPD
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Overview

  • Supplier
    Biorbyt
  • Product Name
    PRD Rabbit mAb
  • Delivery Days Customer
    10
  • Applications
    Western Blot
  • Applications Supplier
    WB 1:500~1:2000 WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5184
  • Target name
    PEPD
  • Target description
    peptidase D
  • Target synonyms
    aminoacyl-L-proline hydrolase; imidodipeptidase; PROLIDASE; proline dipeptidase; testicular tissue protein Li 138; xaa-Pro dipeptidase; X-Pro dipeptidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12955
  • Protein Name
    Xaa-Pro dipeptidase
  • Scientific Description
    Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PEPD using anti-PEPD antibody (A03417-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEPD antigen affinity purified polyclonal antibody (Catalog # A03417-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEPD at approximately 60 kDa. The expected band size for PEPD is at 55 kDa.
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