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WB analysis of A549 lysate using GTX89551 PRDM11 antibody, C-term. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of A549 lysate using GTX89551 PRDM11 antibody, C-term. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of A549 lysate using GTX89551 PRDM11 antibody, C-term. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer

PRDM11 antibody, C-term

GTX89551
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPRDM11
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Overview

  • Supplier
    GeneTex
  • Product Name
    PRDM11 antibody, C-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 2-3microg/ml. IHC-P: 5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56981
  • Target name
    PRDM11
  • Target description
    PR/SET domain 11
  • Target synonyms
    PFM8; PR domain-containing protein 11; PR-domain containing protein 11
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9NQV5
  • Protein Name
    PR domain-containing protein 11
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: A549 whole cell lysate All lanes: PRDM11 antibody at 5.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 54 kDa Observed band size: 58 kDa
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Figure 1. Western blot analysis of PRDM11 using anti-PRDM11 antibody (A11092-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM11 antigen affinity purified polyclonal antibody (Catalog # A11092-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM11 at approximately 58 kDa. The expected band size for PRDM11 is at 58 kDa.
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