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Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: PRDM5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 70, 13, 59 kDa Observed band size: 74 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: PRDM5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 70, 13, 59 kDa Observed band size: 74 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: PRDM5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 70, 13, 59 kDa Observed band size: 74 kDa

PRDM5 Antibody

CSB-PA868292LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetPRDM5
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRDM5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11107
  • Target name
    PRDM5
  • Target description
    PR/SET domain 5
  • Target synonyms
    BCS2, PFM2, PR domain zinc finger protein 5, PR domain 5, PR domain containing 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQX1
  • Protein Name
    PR domain zinc finger protein 5
  • Scientific Description
    Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes. May regulate the expression of proteins involved in extracellular matrix development and maintenance, including fibrillar collagens, such as COL4A1 and COL11A1, connective tissue components, such as HAPLN1, and molecules regulating cell migration and adhesion, including EDIL3 and TGFB2. May caused G2/M arrest and apoptosis in cancer cells.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRDM5 using anti-PRDM5 antibody (A06736-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat ovary tissue lysates, Lane 3: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM5 antigen affinity purified polyclonal antibody (Catalog # A06736-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM5 at approximately 75KD. The expected band size for PRDM5 is at 75KD.
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