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Western Blot analysis of Jurkat cells using PREP Polyclonal Antibody
Western Blot analysis of Jurkat cells using PREP Polyclonal Antibody
Western Blot analysis of Jurkat cells using PREP Polyclonal Antibody

PREP Antibody

CSB-PA004861
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPREP
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Overview

  • Supplier
    Cusabio
  • Product Name
    PREP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5550
  • Target name
    PREP
  • Target description
    prolyl endopeptidase
  • Target synonyms
    PE, PEP, prolyl endopeptidase, dJ355L5.1 (prolyl endopeptidase), post-proline cleaving enzyme, prolyl oligopeptidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N6D4
  • Protein Name
    Prolyl endopeptidase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Prolyl Endopeptidase/PREP using anti-Prolyl Endopeptidase/PREP antibody (A00298-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prolyl Endopeptidase/PREP antigen affinity purified polyclonal antibody (Catalog # A00298-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prolyl Endopeptidase/PREP at approximately 81 kDa. The expected band size for Prolyl Endopeptidase/PREP is at 81 kDa.
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