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Western Blot Positive WB detected in: Mouse brain tissue All lanes: PREX2 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 183, 172, 113 kDa Observed band size: 183, 113 kDa
Western Blot Positive WB detected in: Mouse brain tissue All lanes: PREX2 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 183, 172, 113 kDa Observed band size: 183, 113 kDa
Western Blot Positive WB detected in: Mouse brain tissue All lanes: PREX2 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 183, 172, 113 kDa Observed band size: 183, 113 kDa

PREX2 Antibody

CSB-PA741122LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPREX2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PREX2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80243
  • Target name
    PREX2
  • Target description
    phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2
  • Target synonyms
    DEP.2, DEPDC2, P-REX2, PPP1R129, phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein, DEP domain-containing protein 2, PtdIns(3,4,5)-dependent Rac exchanger 2, protein phosphatase 1, regulatory subunit 129
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ70Z35
  • Protein Name
    Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
  • Scientific Description
    Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PREX2 using anti-PREX2 antibody (A06715-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skeletal muscle lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PREX2 antigen affinity purified polyclonal antibody (Catalog # A06715-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PREX2 at approximately 183 kDa. The expected band size for PREX2 is at 183 kDa.
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Immunohistochemistry analysis in human placenta and small intestine tissues using HPA015234 antibody. Corresponding PREX2 RNA-seq data are presented for the same tissues.
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