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Primary Antibodies

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Western Blot Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate All lanes: HIST1H1C antibody at 1:100 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Product group Antibodies
Cusabio
Mono-methyl-HIST1H1C (K45) AntibodyCSB-PA010378OA45ME1HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH1-2
  • SizePrice
Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate All lanes: HIST1H1C antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Product group Antibodies
Cusabio
Di-methyl-HIST1H1C (K45) AntibodyCSB-PA010378OA45ME2HU
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH1-2
  • SizePrice
IHC image of CSB-PA010378OA45nme1HU diluted at 1:20 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
HIST1H1C (Ab-45) AntibodyCSB-PA010378OA45NME1HU
ApplicationsImmunoFluorescence, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetH1-2
  • SizePrice
Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Rat kidney tissue, Mouse liver tissue, Mouse kidney tissue All lanes: HIST1H1C antibody at 1:100 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Product group Antibodies
Cusabio
HIST1H1C (Ab-45) AntibodyCSB-PA010378OA45NME2HU
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetH1-2
  • SizePrice
Immunocytochemistry analysis of CSB-PA010378OA51hibHU diluted at 1:25 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
2-hydroxyisobutyryl-HIST1H1C (K51) AntibodyCSB-PA010378OA51HIBHU
ApplicationsELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH1-2
  • SizePrice
IHC image of CSB-PA010378OA51nhibHU diluted at 1:20 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
HIST1H1C (Ab-51) AntibodyCSB-PA010378OA51NHIBHU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetH1-2
  • SizePrice
IHC image of CSB-PA010378OA62ncrHU diluted at 1:20 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
HIST1H1C (Ab-62) AntibodyCSB-PA010378OA62NCRHU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetH1-2
  • SizePrice
IHC image of CSB-PA010378OA62nsucHU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
HIST1H1C (Ab-62) AntibodyCSB-PA010378OA62NSUCHU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetH1-2
  • SizePrice
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010378OA74acHU at 1:7.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
Acetyl-HIST1H1C (K74) AntibodyCSB-PA010378OA74ACHU
ApplicationsImmunoFluorescence, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
TargetH1-2
  • SizePrice
Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5microg anti-HIST1H1C (CSB-PA010378OA74forHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.
Product group Antibodies
Cusabio
Formyl-HIST1H1C (K74) AntibodyCSB-PA010378OA74FORHU
ApplicationsChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
TargetH1-2
  • SizePrice
IHC image of CSB-PA010378OA74nhibHU diluted at 1:20 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
HIST1H1C (Ab-74) AntibodyCSB-PA010378OA74NHIBHU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetH1-2
  • SizePrice
Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5microg anti-HIST1H1C (CSB-PA010378OA80hibHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.
Product group Antibodies
Cusabio
2-hydroxyisobutyryl-HIST1H1C (K80) AntibodyCSB-PA010378OA80HIBHU
ApplicationsChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
TargetH1-2
  • SizePrice
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