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ProductsBack/PRKAA1 / AMPK Alpha 1 Antibody (clone 2B7)

PRKAA1 / AMPK Alpha 1 Antibody (clone 2B7)

LS-C169322
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRKAA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PRKAA1 / AMPK Alpha 1 Antibody (clone 2B7)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), IHC, IHC-P (1:200 - 1:1000), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2B7
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5562
  • Target name
    PRKAA1
  • Target description
    protein kinase AMP-activated catalytic subunit alpha 1
  • Target synonyms
    5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; ACACA kinase; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK alpha 1; AMPK subunit alpha-1; AMPK, alpha, 1; AMPKa1; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; tau-protein kinase PRKAA1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMPK alpha 1 using anti-AMPK alpha 1 antibody (A00994-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1 antigen affinity purified polyclonal antibody (Catalog # A00994-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1 at approximately 64 kDa. The expected band size for AMPK alpha 1 is at 64 kDa.
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