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Western Blot analysis of A549 cells using Glucosidase IIbeta Polyclonal Antibody
Western Blot analysis of A549 cells using Glucosidase IIbeta Polyclonal Antibody
Western Blot analysis of A549 cells using Glucosidase IIbeta Polyclonal Antibody

PRKCSH Antibody

CSB-PA002713
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetPRKCSH
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRKCSH Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5589
  • Target name
    PRKCSH
  • Target description
    PRKCSH beta subunit of glucosidase II
  • Target synonyms
    80K-H, AGE-R2, G19P1, GIIB, GIIbeta, GluIIbeta, PCLD, PCLD1, PKCSH, PLD1, VASAP-60, glucosidase 2 subunit beta, AGE-binding receptor 2, advanced glycation end-product receptor 2, glucosidase II subunit beta, hepatocystin, protein kinase C substrate 60.1 kDa protein heavy chain, protein kinase C substrate 80K-H, protein kinase C substrate, 80 Kda protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14314
  • Protein Name
    Glucosidase 2 subunit beta
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRKCSH using anti-PRKCSH antibody (A04992-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCSH antigen affinity purified polyclonal antibody (Catalog # A04992-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCSH at approximately 90 kDa. The expected band size for PRKCSH is at 59,80-90 kDa.
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