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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA018727LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA018727LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA018727LA01HU at dilution of 1:100

PRLR Antibody

CSB-PA018727LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRLR
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRLR Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5618
  • Target name
    PRLR
  • Target description
    prolactin receptor
  • Target synonyms
    HPRL; hPRL receptor; hPRLrI; MFAB; prolactin receptor; RI-PRLR; secreted prolactin binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16471
  • Protein Name
    Prolactin receptor
  • Scientific Description
    This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Paraformaldehyde-fixed, paraffin embedded mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with PRLR Polyclonal Antibody (bs-6445R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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Figure 1. Western blot analysis of PRLR using anti-PRLR antibody (PB9782). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody (Catalog # PB9782) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRLR at approximately 65, 95 kDa. The expected band size for PRLR is at 70 kDa.
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WB analysis of extracts from 293T cells expressing human PRLR protein using GTX50723 Prolactin Receptor antibody.
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