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Western blot All lanes: PRMT3 antibody at 7ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 53 kDa Observed band size: 53 kDa
Western blot All lanes: PRMT3 antibody at 7ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 53 kDa Observed band size: 53 kDa
Western blot All lanes: PRMT3 antibody at 7ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 53 kDa Observed band size: 53 kDa

PRMT3 Antibody

CSB-PA018733LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRMT3
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRMT3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10196
  • Target name
    PRMT3
  • Target description
    protein arginine methyltransferase 3
  • Target synonyms
    heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3; HMT1 hnRNP methyltransferase-like 3; HRMT1L3; protein arginine N-methyltransferase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60678
  • Protein Name
    Protein arginine N-methyltransferase 3
  • Scientific Description
    Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRMT3 using anti-PRMT3 antibody (A05694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT3 antigen affinity purified polyclonal antibody (Catalog # A05694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT3 at approximately 60-70 kDa. The expected band size for PRMT3 is at 60 kDa.
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