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PRMT6 Antibody (clone 4G2)

LS-C169197
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPRMT6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PRMT6 Antibody (clone 4G2)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4G2
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55170
  • Target name
    PRMT6
  • Target description
    protein arginine methyltransferase 6
  • Target synonyms
    heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase-like 6; HRMT1L6; protein arginine N-methyltransferase 6
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRMT6 using anti-PRMT6 antibody (A02924-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT6 antigen affinity purified polyclonal antibody (Catalog # A02924-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT6 at approximately 40 kDa. The expected band size for PRMT6 is at 40 kDa.
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