Pro Ligation-Free Cloning Kit (50 reactions)

Applied Biological Materials

Pro Ligation-Free Cloning Kit (50 reactions)

16

Research Use Only

The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability. abms Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs. Key Kit Features: One-step cloning: No more time-consuming, multi-step traditional cloning that depnds on available restrict sites or compatible ends Scar-free cloning: No unwanted/extra base pairs in the inserts Easy-to-customize: Clone in promoter, gene, tag, multi-fragment or whiole vector constructs in one step Ready for downstream applications: Ligase-free reaction, no gel extraction or column purification steps needed Robust assembly: Better performance for difficult, multi-fragment assemblies such as CRISPR multiplex sgRNA vector assembly Increased thermostability: High colony yields Efficient, low-cost alternative to Gibson Assembly

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